Prime Minister Scott Morrison choked back tears discussing what he described as a “shameful” month before turning and clashing with the media.
Canberra has been rocked by sex claims and rape allegations in recent weeks.
Issues emerged when former staffer Brittany Higgins came forward to allege she was raped in her workplace by a colleague in 2019.
Stream your local 7NEWS free on 7plus 
On Monday, further claims of misconduct at Parliament House were aired by Channel 10.
In the report, images and videos of government staffers performing graphic sex acts in the Prayer Room were aired by a whistleblower.
Morrison, in a press conference on Tuesday, described the latest report as “disgraceful”.
“All of this has been shocking, it has been disgraceful,” he said.
“It has been a month of such reports. Indeed, reports involving the conduct of staff and, of Coalition government members and ministers.”
His voice broke as he spoke of discussing the allegations with his family.
“Criticise me if you like for speaking about my daughters, but they are the centre of my life,” he said.
“My wife is the centre of my life.”
But the press conference took a turn when Morrison accused a journalist of throwing stones in “glass houses”.
When asked whether his job was in jeopardy following the wave of allegations, Morrison responded: “I will let you editorialise as you like, but if anyone in this room wants to offer up the standards in their own workplaces as comparison I would invite you to do so.”
The journalist suggested that conditions were dire in Parliament House.
Morrison responded by referencing an allegation that he says is the subject of a complaint in the journalist’s organisation.
“And that matter is being pursued by your own HR department.”
The journalist, however, said he did not know of the claim.
“You are not aware of it? So let’s not all of us who sit in glass houses here start getting into that.”
Morrison continued to argue that “no single individual can be overwatch on every single inch of this place every second of the day”.
“We must get these house in order,” he said.
“We must put the politics aside of these things, and we must recognise this problem, we must fix it.”
He said the allegation aired on Channel 10 took place “long before” he was made prime minister in 2018.
Senior ministers, meanwhile, have expressed their disgust after the coalition staffer was sacked for performing a lewd sex act inside a federal Liberal MP’s office.
Finance Minister Simon Birmingham, whose department manages much of Parliament House, said the conduct was revolting.
Channel 10 broadcast obtained images and videos filmed inside parliament involving four coalition staff members.
“Amongst a small number, there is clearly a disgusting and unacceptable culture, and we have to weed it out,” Senator Birmingham told the ABC on Tuesday.
“One employee was dismissed last night, and if others are identified as being engaged in similar activity, they will be dismissed as well.”